Download ebook Biophysics and the Challenges of Emerging Threats
Download ebook Biophysics and the Challenges of Emerging Threats
Biophysics and the Challenges of Emerging Threats.cJoseph D Puglisi
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Author: Joseph D Puglisi
Number of Pages: 186 pages
Published Date: 05 Jun 2009
Publisher: Springer
Publication Country: Dordrecht, Netherlands
Language: English
ISBN: 9789048123674
File size: 16 Mb
File Name: Biophysics.and.the.Challenges.of.Emerging.Threats.pdf
Download Link: Biophysics and the Challenges of Emerging Threats
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Single-molecule techniques eliminate ensemble averaging, thus revealing transient or rare species in heterogeneous systems [1-3]. These approaches have been employed to probe myriad biological phenomena, including protein and RNA folding [4-6], enzyme kinetics [7, 8], and even protein biosynthesis [1, 9, 10]. In particular, immobilization-based fluorescence te- niques such as total internal reflection fluorescence microscopy (TIRF-M) have recently allowed for the observation of multiple events on the millis- onds to seconds timescale [11-13]. Single-molecule fluorescence methods are challenged by the instability of single fluorophores. The organic fluorophores commonly employed in single-molecule studies of biological systems display fast photobleaching, intensity fluctuations on the millisecond timescale (blinking), or both. These phenomena limit observation time and complicate the interpretation of fl- rescence fluctuations [14, 15]. Molecular oxygen (O) modulates dye stability. Triplet O efficiently 2 2 quenches dye triplet states responsible for blinking. This results in the for- tion of singlet oxygen [16-18]. Singlet O reacts efficiently with organic dyes, 2 amino acids, and nucleobases [19, 20]. Oxidized dyes are no longer fluor- cent; oxidative damage impairs the folding and function of biomolecules. In the presence of saturating dissolved O , blinking of fluorescent dyes is sup- 2 pressed, but oxidative damage to dyes and biomolecules is rapid. Enzymatic O -scavenging systems are commonly employed to ameliorate dye instability. 2 Small molecules are often employed to suppress blinking at low O levels.
Read online Biophysics and the Challenges of Emerging Threats Buy and read online Biophysics and the Challenges of Emerging Threats Download Biophysics and the Challenges of Emerging Threats ebook, pdf, djvu, epub, mobi, fb2, zip, rar, torrent Download to iPad/iPhone/iOS, B&N nook Biophysics and the Challenges of Emerging Threats ebook, pdf, djvu, epub, mobi, fb2, zip, rar, torrent
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A Genuine Blessing
Biophysics and the Challenges of Emerging Threats.cJoseph D Puglisi
Author: Joseph D Puglisi
Number of Pages: 186 pages
Published Date: 05 Jun 2009
Publisher: Springer
Publication Country: Dordrecht, Netherlands
Language: English
ISBN: 9789048123674
File size: 16 Mb
File Name: Biophysics.and.the.Challenges.of.Emerging.Threats.pdf
Download Link: Biophysics and the Challenges of Emerging Threats
---------------------------------------------------------------
Single-molecule techniques eliminate ensemble averaging, thus revealing transient or rare species in heterogeneous systems [1-3]. These approaches have been employed to probe myriad biological phenomena, including protein and RNA folding [4-6], enzyme kinetics [7, 8], and even protein biosynthesis [1, 9, 10]. In particular, immobilization-based fluorescence te- niques such as total internal reflection fluorescence microscopy (TIRF-M) have recently allowed for the observation of multiple events on the millis- onds to seconds timescale [11-13]. Single-molecule fluorescence methods are challenged by the instability of single fluorophores. The organic fluorophores commonly employed in single-molecule studies of biological systems display fast photobleaching, intensity fluctuations on the millisecond timescale (blinking), or both. These phenomena limit observation time and complicate the interpretation of fl- rescence fluctuations [14, 15]. Molecular oxygen (O) modulates dye stability. Triplet O efficiently 2 2 quenches dye triplet states responsible for blinking. This results in the for- tion of singlet oxygen [16-18]. Singlet O reacts efficiently with organic dyes, 2 amino acids, and nucleobases [19, 20]. Oxidized dyes are no longer fluor- cent; oxidative damage impairs the folding and function of biomolecules. In the presence of saturating dissolved O , blinking of fluorescent dyes is sup- 2 pressed, but oxidative damage to dyes and biomolecules is rapid. Enzymatic O -scavenging systems are commonly employed to ameliorate dye instability. 2 Small molecules are often employed to suppress blinking at low O levels.
Read online Biophysics and the Challenges of Emerging Threats Buy and read online Biophysics and the Challenges of Emerging Threats Download Biophysics and the Challenges of Emerging Threats ebook, pdf, djvu, epub, mobi, fb2, zip, rar, torrent Download to iPad/iPhone/iOS, B&N nook Biophysics and the Challenges of Emerging Threats ebook, pdf, djvu, epub, mobi, fb2, zip, rar, torrent
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